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Experiment PP216 is an experiment in a larger project the Vault 101's vault physician James performed in 2274. James, according to his notes, "borrowed" some water chips and rerouted the Clinic's power supply in order to perform unsanctioned personal experiments, most likely related to Project Purity, given the "PP" designation. The results in the notes are vague, but optimistic. He also notes that this is the first time that his assistant Jonas Palmer had helped in him in this project.

The Lone Wanderer reads the notes for this experiment from the Medical Data System in their Dad's terminal located in the Physician's Office on August 3, 2274.