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The effects of Rad-X are stackable up to two subsequent doses, but taking an immediate third dose of Rad-X has no effect. This is due to the fact that in the original Fallout, taking two doses of Rad-X granted 100% radiation resistance, enough to survive the most irradiated place on the map for several hours.
Despite having no harmful effects, in Fallout Tactics it is possible to overdose if taken too quickly or alongside other chems, depending on Endurance.